Other

Part:BBa_K1207000:Design

Designed by: Jeremiah Miller   Group: iGEM13_OU-Norman_OK   (2013-09-12)


Gram positive origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cloning of this part was relatively straight-forward. No special considerations were required.


Source

This part was cloned from the plasmid pIKM1. Its original source was B. subtilis.

References

Mai, V., W. W. Lorenz, and J. Wiegel. Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiology Letters. 1997. 148:163-167.